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How to make Mushroom Spore Syringes

Spore SyringeOnce you have collected spore prints from your magic mushrooms, it‘s time to make spore syringes. Essentially, the syringe consists of a mix of sterile water and spores that are used to start the germination of your next batch of shrooms.

Hydration of spores is an important process before mushroom breeding can begin, because dry spores are very unlikely to germinate. That is one reason why spore syringes are convenient to use; the spores are naturally hydrated and ready to go.

Buy Spore Syringes

To create a spore syringe you will need:

• A spore print
• Water
• Sterile empty syringes
• A glass narrow necked flask or beaker
• Tin foil
• A pressure cooker
• A scalpel
Tweezers
• A gas hob or burner

Always remember to work as clean as possible. If available, work under a flow hood.

The process:

1. Start out by sterilising your water and glass beaker. Fill the beaker about half way with water and then cover the top with tin foil so no air can get in.

2. Place the bottle in your pressure cooker and heat it until it reaches 15psi. Once you have reached pressure, lower the temperature so that it is maintained for about 30 minutes.

3. Turn off the heat completely after 30 minutes and allow the water to cool to room temperature. That‘s very important, if you proceed while the water is still hot, you run a huge risk of killing the spores. It should take a few hours to completely cool, but it‘s easiest to let it sit over night.

4. Now you are ready to make the spore syringes. Sterilise your tweezers and scalpel in a flame.

5. Remove the tin foil from you beaker.

6. With your tweezers, take the spore print from its storage and hold it over the opening of the beaker.

7. With the scalpel in hand, carefully scrape some of the spores into the water.

8. You should fill you syringes instantly - place the tip of your first syringe in the water and fill it up. It is a good idea to empty the syringe back into the beaker, and repeat this process a couple of times. This will ensure there is a good spread of spores throughout the water – do this every time you add more spores.

9. Now that you have a filled syringe you need to leave it at room temperature for 2-3 days so that the spores can fully hydrate.

10. After a few days you can store your syringes in an airtight zip lock bag, or in a fridge for future use. They‘re remain viable for 2-6 months.

That’s it, now you know how to make your own spore syringes. There are many variations to this method, and more advanced equipment and containers can be used to increase the chances of your mixture staying sterile, but this is a good solid foundation that will yield very good rates of success.

 

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