How to make liquid culture

Not to be confused with spore syringes, liquid cultures contain live mycelium (the root-like vegetative structure of mushrooms). Offering faster inoculation and colonisation, liquid cultures can streamline home mushroom grows and are perfect for mycologists of all skill levels.

If you want more information on the unique properties and benefits of liquid cultures as well as how to use liquid culture to grow mushrooms, consider our detailed guides. Otherwise, jump right into our liquid culture recipe below!

Ingredients & equipment

Making liquid culture might seem daunting, especially to beginner mycologists. However, it’s simple: with a few basic ingredients, some household equipment, and the fool-proof liquid mushroom culture recipe below, you’ll be growing your own mushrooms in no time!

Note that some mushroom liquid culture recipes use a colonised plate of agar. While this technique is very successful and popular, we recommend the following recipe (which uses sterile spore syringes, sugar, and water) for its simplicity and low margin for error/contamination.

Ingredients:

• Distilled water
• Honey, light malt extract, or corn syrup
• Spore syringes containing spores of the mushroom species you plan to cultivate

Equipment:

• Glass jar
• Sterile gloves
• Isopropyl alcohol
• Pressure cooker or oven (for sterilising your jars)
• Magnetic stirrer/mixer (optional) or a sterile metal spoon (for stirring your liquid culture)

Penis Envy Liquid Culture Syringe (Acid Shroomz)

(99)

(99)

Penis Envy Liquid Culture Syringe (Acid Shroomz)

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Do not use regular sugar to make liquid culture

Unfortunately, regular sugar (sucrose) isn’t suitable for making liquid culture because fungi do not as easily digest it as simpler sugars such as malt extract or honey, hence the following honey/LME liquid culture recipe. Moreover, sugar doesn’t contain any of the beneficial trace elements like vitamins, minerals, and amino acids that help mycelium thrive. Meanwhile, bacteria like Bacillus or Lactobacillus rapidly digest sucrose, meaning there’s an increased risk of contamination when using it to make liquid culture.

Why you need a spore syringe to make liquid culture

Spore syringes are essential for making your own liquid culture as they contain the spores of the mushrooms you’ll be cultivating. After being injected into a sterile sugar-water mixture, the spores will germinate and form mycelium: the vegetative part of a fungus that, in optimal conditions, produces the fruiting bodies that we know as mushrooms.

The easiest way to make liquid culture from spores is to use our pre-made spore syringes. Spore syringes come in various forms, but ready-to-use syringes are by far the most convenient and beginner-friendly option. Zamnesia Magic Mushroom Spore Syringes are prepared in sterile conditions to help you avoid contamination and ensure excellent harvests every time, straight out of the box.

While most users prefer the simplicity of ready-made syringes, some advanced growers may choose to prepare their own spore syringe from a spore print. This approach offers more control over the process, but demands sterile conditions and a good understanding of mushroom cultivation.
For more information on how to make your own magic mushroom spore syringe, check out our blog: how to make a magic mushroom spore syringe.

Instructions

With your ingredients and equipment assembled, it's finally time to start making your liquid culture. Follow each of the steps below with care, and you're sure to achieve good results.

1. Sterilise your workspace and tools

Good sterile technique is the backbone of all aspects of magic mushroom cultivation, including making liquid culture. Wash and sanitise your hands, wipe down your workspace and all the equipment listed above using isopropyl alcohol, then put on sterile gloves. Contamination is the most common pitfall of home mushroom growing, and it's important to take the necessary precautions right from the get-go.

2. Fill your mycelium growing jar

Now that you have a clean surface to work on, it's time to prepare the medium and container you'll be growing your liquid culture in. To do this, start by filling your glass jar with 400 ml of distilled water (chlorinated water can inhibit mycelium growth, and rainwater may contain bacteria or pathogens that lead to contamination). To the water, add 16 ml of honey (you can also make liquid culture with Karo/corn syrup or malt extract).

These substances contain simple carbs that'll feed your mycelium and provide it with the energy it needs to grow. Make sure to stir the mixture gently using a sterile metal spoon, then loosely cover the jar with aluminium foil.

3. Sterilise your jar

Next, you'll need to sterilise your mycelium growing jar to further prevent contamination. We recommend the following ways to do this:

• a) Place the jar and lid in a pressure cooker with 100 ml of water. Once your pressure cooker reaches 15 psi, heat the jars for 10 minutes.
• b) Place the jar and lid in a preheated oven at 180°C and heat for 60 minutes.

Note: Do not close your jars when sterilising them. They will explode.

4. Inoculate your jar

Remove your jar from the oven or pressure cooker and let it cool completely. A hot mixture will kill your spores and keep them from germinating and growing into mycelium. Once the mixture has cooled, inject at least 1–2 ml of spores from your syringe into the water-honey mixture. For the best results, use a  our sterile spore syringe.

If you're not using a pre-made sterile syringe, make sure to sterilise whatever syringe you're using by heating its tip in a flame until it glows red.

5. Incubate your spores

Close the jar and store it in a dry, dark place at a stable temperature of 18–20°C. Light and abrupt temperature shifts will inhibit your spores from germinating and could hinder liquid culture development. Make sure to use a thermometer to track the temperature in your incubation space, and consider using a heat mat or space heater if necessary to bring temperatures up to where they should be. Wait at least 2–3 days for the spores to germinate and begin colonising the culture.

6. Oxygenate your liquid culture

Oxygenating your liquid culture is key to supporting its growth. Do this by shaking or stirring and opening your jar once daily (always in a sterile environment). The shaking/stirring will help break up large clumps of mycelium, which keeps it growing evenly throughout the entire mixture. Meanwhile, opening your jar will also allow for important gas exchanges to occur. To stir your mixture, use either a sterile metal spoon or a magnetic stirrer/mixer (these help minimise contamination).

7. Be patient

Wait 10–20 days for your culture to mature. Colonisation timelines vary depending on the mushroom species you’re cultivating.

How do I know if my liquid culture is ready to use?

Healthy liquid culture will contain white mycelium that forms into fine threads or snowflake-like clumps. If your liquid culture doesn’t develop any mycelium after 14–21 days, it may not have inoculated properly. If your homemade liquid culture shows signs of contamination (green/black mould or a foul smell), discard it immediately.

Cultivate in confidence with your own homemade liquid culture!

Liquid cultures harness the power of live mycelium to produce excellent mushroom harvests in express time. With good sterile technique and the best liquid culture recipe we covered above, you can learn how to make a liquid culture at home with ease.

 If you're new to growing magic mushrooms, use one of our ready-made liquid culture syringes. Alternatively, if you have some experience, consider ordering a spore syringe and turning it into an active, fast-growing culture yourself! Once you've got your liquid culture, make sure to learn how to use it, too. And remember, Zamnesia's Shroomshop is you're one-stop shop for everything you need to grow potent magic mushrooms at home!

Steven Voser

Steven Voser is an independent cannabis journalist with over 6 years of experience writing about all things weed; how to grow it, how best to enjoy it, and the booming industry and murky legal landscape surrounding it.

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